Thermo Fisher Scientific MYC Epitope Tag Polyclonal Antibody, Biotin
MYC epitope tag를 인식하는 rabbit polyclonal antibody로, Biotin conjugation되어 Western blot, IHC, ELISA, IP 등 다양한 어플리케이션에 적합. 인간 c-Myc 태그 단백질 검출에 유용하며, 높은 특이성과 안정성을 제공. 연구용으로만 사용 가능.
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Thermo Fisher Scientific MYC Epitope Tag Polyclonal Antibody, Biotin
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (IHC) | 1:1,000–1:5,000 |
| ELISA | 1:100,000–1:500,000 |
| Immunoprecipitation (IP) | 1:2,000–1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Purified from rabbit serum immunized with Myc epitope tag peptide E-Q-K-L-I-S-E-E-D-L conjugated to KLH using maleimide. Sequence corresponds to aa 410–419 of human c-Myc. |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to reconstitution.
- For extended storage, aliquot and freeze at -20°C or below.
- Avoid repeated freeze/thaw cycles.
- Centrifuge if solution is not clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
This affinity-purified antibody is directed against human c-Myc and detects overexpressed proteins containing the Myc epitope tag.
It recognizes the Myc-tag sequence (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) fused to either the amino- or carboxy-termini of targeted proteins in transfected or transformed cells.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Rabbit Serum.
Target Information
Myc is a proto-oncogene encoding a nuclear phosphoprotein involved in cell cycle progression, apoptosis, and cellular transformation.
It forms a heterodimer with transcription factor MAX, binding to the E-box DNA consensus sequence to regulate target gene transcription.
Myc amplification is frequently observed in various human cancers.
Translocations involving Myc are associated with Burkitt lymphoma and multiple myeloma.
Translation may initiate from both upstream non-AUG (CUG) and downstream AUG start sites, producing two isoforms with distinct N-termini.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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