Thermo Fisher Scientific Phosphothreonine Polyclonal Antibody, Biotin

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

-

ELISA (ELISA)

1:5,000

-

Product Specifications

Species Reactivity

Chemical

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PhosphoThreonine affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with phosphothreonine conjugated KLH.

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 50% glycerol, 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store at 4°C prior to opening.

This product is stable for several weeks at 4°C as an undiluted liquid (dilute only prior to immediate use).

For extended storage aliquot contents and freeze at -20°C or below.

Target Information

Phosphothreonine is a phosphoamino acid, the phosphorylated ester of threonine. There are three amino acids that are typically phosphorylated in eukaryotes: serine, threonine, and tyrosine. Threonine residues in endogenous proteins undergo phosphorylation through the action of a threonine kinase. Small amounts of free phosphothreonine can be detected in urine Antibodies against phosphothreonine can be used as an identifier in the analysis of peptides, proteins, and enzymes. [National Center for Biotechnology Information.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.