Thermo Fisher Scientific IRF4 Monoclonal Antibody (3E4), Alexa Fluor 700, eBioscience
IRF4 단백질을 인식하는 3E4 클론의 Rat IgG1 단일클론 항체로, Alexa Fluor 700 형광체 결합 제품입니다. 인간 및 마우스 시료에 반응하며 Flow Cytometry에 최적화되어 있습니다. B세포, T세포, 대식세포 기능 연구에 적합합니다.
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Thermo Fisher Scientific IRF4 Monoclonal Antibody (3E4), Alexa Fluor 700, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | 1 publication |
| Flow Cytometry (Flow) | 0.25 µg/test | 15 publications |
| ELISA | - | 1 publication |
| ChIP assay | - | 1 publication |
Product Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), Alexa Fluor™ 700 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3E4 |
| Conjugate | Alexa Fluor™ 700 |
| Excitation / Emission Max | 696 / 719 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2848475 |
Available Formats
- Unconjugated
- Brilliant Violet 480
- Brilliant Violet 650
- eFluor 450
- eFluor 660
- FITC
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Custom conjugation available
Product Specific Information
The monoclonal antibody 3E4 reacts with human and mouse interferon regulatory factor 4 (IRF4), a 52 kDa transcription factor involved in B cell, T cell, and macrophage function.
- In B cells, IRF4 is highly expressed in mature plasma cells and regulates differentiation and transcription of genes such as Prdm1 (Blimp1).
- In T cells, IRF4 contributes to Treg, Th2, Th9, and Th17 development and function, inducing IL-10 expression.
- IRF4 also plays a role in macrophage polarization and is upregulated in various hematologic malignancies.
Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Intracellular staining of stimulated human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00). Recommended ≤0.25 µg/test in 100 µL final volume. Cell number: 10⁵–10⁸ cells/test. Optimize titration for best results.
Fluorochrome Information:
Alexa Fluor 700 emits at 723 nm and is excited by red laser (633–647 nm). Use 685 LP mirror and 710/20 band pass filter.
Excitation: 633–647 nm; Emission: 723 nm; Laser: Red.
Target Information
The IRF (interferon regulatory factor) family are transcription factors with a tryptophan pentad repeat DNA-binding domain, regulating interferon response and interferon-inducible genes.
IRF4 is lymphocyte-specific and negatively regulates TLR signaling, influencing innate and adaptive immunity. A chromosomal translocation t(6;14)(p25;q32) involving IRF4 and IgH may cause multiple myeloma. Alternative splicing variants exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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