Thermo Fisher Scientific CD309 (FLK1) Monoclonal Antibody (Avas12a1), APC, eBioscience
Mouse FLK1(VEGFR2) 인식 Rat 모노클로날 항체로 APC 형광 표지. Flow cytometry에 최적화되어 있으며 0.5 µg/test 사용 권장. 혈관내피세포 발달 연구에 적합하며 4°C 암소 보관.
- 판매단위
- pk
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Thermo Fisher Scientific CD309 (FLK1) Monoclonal Antibody (Avas12a1), APC, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.5 µg/test | View 39 publications |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| Category | Specification |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | Avas12a1 |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_657865 |
Available Formats
- Unconjugated
- Alexa Fluor 700
- Biotin
- PE
- Super Bright 436
- Super Bright 600
- Request custom conjugation
Product Specific Information
The Avas12a1 monoclonal antibody reacts with mouse Flk-1 (VEGFR2), a receptor tyrosine kinase involved in vascular endothelial tissue development. It is expressed on endothelial cells during embryonic stages and in some adult endothelial tissues.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of bEnd.3 cells. Recommended ≤0.5 µg per test (100 µL final volume). Cell number can range from 10⁵ to 10⁸ cells/test. Titrate for optimal performance.
Excitation / Emission / Laser: 633–647 nm / 660 nm / Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
KDR (FLK1, VEGFR2) is a type III receptor tyrosine kinase that binds vascular endothelial growth factor with high affinity. Expressed early by endothelial precursors, it regulates vascular development. Signaling and trafficking are influenced by Rab GTPase, P2Y purine receptor, integrin αVβ3, and T-cell protein tyrosine phosphatase. Mutations are implicated in infantile capillary hemangiomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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