Thermo Fisher Scientific Histamine H2 Receptor (HRH2) (extracellular) Polyclonal Antibody
HRH2 단백질의 세포외 루프를 인식하는 Rabbit Polyclonal 항체로 Mouse 및 Rat 시료에 반응. Western blot, IHC, Flow cytometry에 적합. 항원 친화 크로마토그래피로 정제된 Lyophilized 형태로 제공되며 안정적인 저장 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Flow Cytometry (Flow) | 1:20 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RNGTRGGNDTFKC, corresponding to amino acids 161–173 of rat HRH2, 2nd extracellular loop |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.8 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Reconstitution: Add 25 µL, 50 µL, or 0.2 mL of double distilled water (DDW), depending on sample size.
Ships as a lyophilized powder at room temperature. Store at -20°C upon arrival.
Reconstituted solution can be stored at 4°C for up to 1 week. For longer storage, aliquot and keep at -20°C.
Avoid multiple freeze/thaw cycles. Centrifuge antibody preparations before use (10,000 x g, 5 min).
Target Information
Histamine H2 Receptor stimulates adenylate cyclase activity and promotes gastric acid release.
It is involved in gastrointestinal motility and intestinal secretion.
Inhibition of HRH2 activity is the basis for treatments of acid-peptic disorders.
The 3’-untranslated region may regulate differential expression.
Histamine H2 receptor expression has been reported in bone marrow, brain, heart, lymph node, nose, placenta, spinal cord, and stomach.
An EST has been isolated from a kidney library.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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