Thermo Fisher Scientific Involucrin (Squamous Cell Terminal Differentiation Marker) Monoclonal Antibody (SY5)

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

-

Immunohistochemistry (PFA fixed) (IHC (PFA))

0.1-0.2 µg/mL

-

Product Specifications

Species Reactivity

Dog, Human, Non-human primate, Pig

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

SY5

Immunogen

Purified involucrin from human keratinocytes if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Involucrin,uniProtId:P07476-1,ncbiNodeId:9606,antigenRange:1-585,antigenLength:585,antigenImageFileName:3713-MSM1-P0_Involucrin_P07476-1_House_mouse.svg,antigenImageFileNamePDP:3713-MSM1-P0_Involucrin_P07476-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

200 µg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4, with 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Does not react with Mouse

Target Information

Involucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures it is expressed by all cells that have left the basal layer. Involucrin expression is abnormal in squamous cell carcinomas and premalignant lesions and is reduced in severe dysplasias of the larynx and cervix. Its gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.