Thermo Fisher Scientific CD223 (LAG-3) Monoclonal Antibody (3DS223H), Brilliant Ultra Violet 563, eBioscience

Thermo Fisher Scientific CD223 (LAG-3) Monoclonal Antibody (3DS223H), Brilliant Ultra Violet 563, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.25 µg)/test

Product Specifications

Additional Formats

• Alexa Fluor 647
• Alexa Fluor 660
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant UV 395, 661, 737, 805
• Brilliant Violet 421, 480, 605, 650, 711, 786
• eFluor 450, 506
• FITC
• NovaFluor Blue 510, Blue 660-120S, Red 700, Yellow 590, Yellow 610, Yellow 700, Yellow 730
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation available

Product Specific Information

This 3DS223H monoclonal antibody recognizes human CD223, also known as Lymphocyte Activation Gene 3 (LAG-3).
LAG-3 is a 70-kDa surface glycoprotein belonging to the Ig superfamily with homology to CD4. It binds to MHC class II with higher affinity than CD4 and is involved in negative regulation of T cell activation and homeostatic proliferation.

Surface expression of LAG-3 is found on activated T cells (including regulatory T cells) and NK cells. CD8+ T cells generally express LAG-3 at higher levels than CD4+ T cells. Coexpression of LAG-3 and CD49b identifies human and mouse Type 1 regulatory T cells (Tr1 cells).

• Recognizes a formaldehyde-fixed epitope
• Reported for use in flow cytometric analysis
• Tested on stimulated normal human peripheral blood cells
• Recommended use: 5 µL (0.25 µg) per test (100 µL final volume)
• Suitable for UV laser-equipped cytometers (Excitation: 350 nm; Emission: 564 nm)

Handling Recommendations:

• Use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific polymer interactions.
• Protect from light to prevent photo-induced oxidation.
• Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency.

BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.

Target Information

LAG-3 is a 70-kDa surface glycoprotein of the Ig superfamily with homology to CD4. It binds MHC class II with high affinity and regulates T cell activation and proliferation. Expressed on activated T cells, regulatory T cells, and NK cells; CD8+ T cells typically show higher expression than CD4+ T cells. Coexpression with CD49b identifies Tr1 cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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