Thermo Fisher Scientific CD170 (Siglec F) Monoclonal Antibody (1RNM44N), Brilliant Ultra Violet 563, eBioscience

Applications

• Flow Cytometry (Flow): Tested at ≤ 0.5 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 700
• Brilliant UV 395
• Brilliant UV 615
• Brilliant UV 737
• Brilliant Violet 480
• Brilliant Violet 605
• Brilliant Violet 711
• eFluor 660
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 800
• NovaFluor Red 685
• NovaFluor Red 710
• NovaFluor Yellow 690
• NovaFluor Yellow 810
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 780
• Request custom conjugation

Product Specific Information

This 1RNM44N monoclonal antibody recognizes mouse CD170, also known as Siglec F.
Siglec F is a cell surface lectin belonging to the Ig superfamily that binds glycoconjugates containing sialic acids. It is expressed primarily on eosinophils and alveolar macrophages, with lower levels on immature myelomonocytic cells.
Mouse Siglec F is a functional ortholog of human Siglec 8 but is not expressed on mast cells.

• Recognizes formaldehyde-fixed epitope
• Reported application: Flow cytometric analysis
• Tested on mouse thioglycolate-elicited peritoneal exudate cells
• Recommended usage: ≤ 0.5 µg/test (100 µL final volume, 10⁵–10⁸ cells/test)
• Titrate for optimal performance

Fluorochrome Information:
Brilliant Ultra Violet™ 563 (BUV563) emits at 564 nm and is designed for cytometers with a 355 nm UV laser.
Protect from light to prevent photo-induced oxidation.

Recommended Buffers:

• Super Bright Complete Staining Buffer (SB-4401-42)
• Brilliant Stain Buffer™ (00-4409-75)

Fixation Options:

• IC Fixation Buffer (00-8222-49)
• 1-step Fix/Lyse Solution (00-5333-54)
  Samples can be stored up to 3 days at 4°C in the dark with minimal fluorescence loss.

Excitation: 350 nm
Emission: 564 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

SIGLEC5 is a putative adhesion molecule mediating sialic-acid dependent binding to cells. It binds both α2,3- and α2,6-linked sialic acids.
Expressed mainly in monocytic/myeloid lineage cells, it is found at high levels in peripheral blood leukocytes, spleen, and bone marrow, and at lower levels in lymph node, lung, appendix, placenta, pancreas, and thymus.
Expressed by monocytes and neutrophils, but absent in early leukemic myelomonocytic cell lines.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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