Thermo Fisher Scientific NFIA Monoclonal Antibody (OTI4G7), TrueMAB

Applications

• Western Blot (WB): 1:2,000 dilution tested

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

NF-1 (also designated CTF) is a family of CCAAT box-binding proteins that stimulate DNA replication and activate transcription.
Human NF-1 mRNA analysis shows two forms of the NF-1 protein arising from alternative splicing of a single NF-1 gene.
NF-1 binds its consensus DNA element as a homodimer via an amino-terminal DNA-binding domain and activates transcription through a proline-rich C-terminal transactivation domain.
It recognizes and binds to the adenovirus type 2 promoter and activates transcription of herpes simplex virus thymidine kinase genes.
NF-1 consensus elements are found in the promoters of numerous eukaryotic genes, including Ha-Ras, alpha-globin, HSP70, GRP78, Histone H1, myelin basic protein, and Xenopus laevis vitellogenin gene promoter.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.