
Thermo Fisher Scientific CDC45 Monoclonal Antibody (OTI4F3), TrueMAB
CDC45 단백질을 인식하는 단클론 항체로, Western blot 및 IHC(Paraffin) 실험에 적합합니다. Human, Mouse, Rat 반응성을 가지며, IgG1 아이소타입입니다. 무보존제, 동결건조 형태로 제공되며 연구용으로 사용됩니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4F3 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1–270 of human CDC45 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step. (Recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882)
Target Information
The CDC45 protein is homologous to Saccharomyces cerevisiae Cdc45, essential for DNA replication initiation.
It forms part of a conserved multiprotein complex with Cdc6/Cdc18, MCMs, and DNA polymerase, crucial for early eukaryotic DNA replication steps.
CDC45 interacts with MCM7 and DNA polymerase alpha and plays a key role in loading DNA polymerase alpha onto chromatin.
Multiple polyadenylation sites are reported for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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