Thermo Fisher Scientific NK1.1 Monoclonal Antibody (PK136), Biotin, eBioscience
Mouse NK1.1 항원을 인식하는 PK136 클론의 단클론 항체로, Biotin으로 결합된 형태. Flow cytometry 및 면역분석에 적합하며, C57BL/6 등 NK1.1 발현 마우스에서 NK 세포 검출에 사용. 0.5 µg/test로 최적화된 농도, 4°C 보관 권장.
- 카탈로그번호
- 13-5941-8x (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific NK1.1 Monoclonal Antibody (PK136), Biotin, eBioscience
Thermo Fisher Scientific NK1.1 Monoclonal Antibody (PK136), Biotin, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.5 µg/test | View 81 publications |
| Functional Assay | - | View 3 publications |
| In vitro Assay (IV) | - | View 1 publication |
| Miscellaneous PubMed | - | View 7 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PK136 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_466803 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 561
- Alexa Fluor 660
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Brilliant UV 395 / 496 / 615 / 661 / 805
- Brilliant Violet 421 / 480 / 605 / 650 / 711 / 786
- eFluor 450
- FITC
- Functional Grade
- PE / PE-Cyanine7 / PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436 / 600 / 645 / 702 / 780
- Request custom conjugation available
Product Specific Information
Description:
PK136 monoclonal antibody reacts with mouse NK1.1 antigen, expressed by natural killer cells and subsets of T cells in NK1.1-positive mouse strains (C57BL, NZB). Strains such as BALB/c, SJL, AKR, CBA, C3H, and A do not express NK1.1 antigen. For NK cell detection in these strains, use the DX5 monoclonal antibody (Product #14-5971).
Simultaneous staining of C57BL/6 spleen cells with PK136 and DX5 reveals coexpression of both markers by a majority of cells, as well as small populations of DX5⁺PK136⁻ and DX5⁻PK136⁺ cells.
Applications Reported: PK136 has been reported for use in flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of C57BL/6 mouse splenocytes, usable at ≤0.5 µg/test. One test defines the amount of antibody staining a cell sample in 100 µL final volume. Recommended to titrate for optimal performance.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
NK1.1 (KLRB1, NKR-P1A, CD161):
NK1.1 refers to Natural Killer (NK) cells, lymphocytes mediating cytotoxicity and cytokine secretion after immune stimulation. The KLRB1 protein is a type II membrane protein of the C-type lectin family, containing extracellular lectin motifs, transmembrane, and cytoplasmic domains. The receptor recognizes Lectin Like Transcript-1 (LLT1) as its functional ligand.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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