Thermo Fisher Scientific NK1.1 Monoclonal Antibody (PK136), Biotin, eBioscience

Thermo Fisher Scientific NK1.1 Monoclonal Antibody (PK136), Biotin, eBioscience™

Applications and Tested Dilution

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 561
• Alexa Fluor 660
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant UV 395 / 496 / 615 / 661 / 805
• Brilliant Violet 421 / 480 / 605 / 650 / 711 / 786
• eFluor 450
• FITC
• Functional Grade
• PE / PE-Cyanine7 / PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436 / 600 / 645 / 702 / 780
• Request custom conjugation available

Product Specific Information

Description:
PK136 monoclonal antibody reacts with mouse NK1.1 antigen, expressed by natural killer cells and subsets of T cells in NK1.1-positive mouse strains (C57BL, NZB). Strains such as BALB/c, SJL, AKR, CBA, C3H, and A do not express NK1.1 antigen. For NK cell detection in these strains, use the DX5 monoclonal antibody (Product #14-5971).

Simultaneous staining of C57BL/6 spleen cells with PK136 and DX5 reveals coexpression of both markers by a majority of cells, as well as small populations of DX5⁺PK136⁻ and DX5⁻PK136⁺ cells.

Applications Reported: PK136 has been reported for use in flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of C57BL/6 mouse splenocytes, usable at ≤0.5 µg/test. One test defines the amount of antibody staining a cell sample in 100 µL final volume. Recommended to titrate for optimal performance.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

NK1.1 (KLRB1, NKR-P1A, CD161):
NK1.1 refers to Natural Killer (NK) cells, lymphocytes mediating cytotoxicity and cytokine secretion after immune stimulation. The KLRB1 protein is a type II membrane protein of the C-type lectin family, containing extracellular lectin motifs, transmembrane, and cytoplasmic domains. The receptor recognizes Lectin Like Transcript-1 (LLT1) as its functional ligand.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.