Thermo Fisher Scientific AID Monoclonal Antibody (mAID-2), Biotin, eBioscience

Thermo Fisher Scientific AID Monoclonal Antibody (mAID-2), Biotin, eBioscience™

Applications and Tested Dilution

Product Specifications

Product Specific Information

The monoclonal antibody mAID-2 recognizes human and mouse AID (gene AICDA), also known as Activation-Induced Cytidine Deaminase.
AID is a 24 kDa RNA-editing enzyme that converts cytosine into uracil and plays a critical role in B cell development, specifically in somatic hypermutation (SHM) and immunoglobulin class switch recombination (CSR).

Mutations in AID are associated with Hyper-IgM Syndrome (type 2), while overexpression correlates with poor prognosis in chronic lymphocytic leukemia/small lymphocytic lymphoma.

AID is mainly expressed by activated mature B cells (germinal centre B cells) and intermediate GC cells (IgD+CD38-CD23-CD71+). It is also found in primary human hepatocellular carcinomas, helicobacter-infected gastric epithelial cells, oocytes, and embryonic stem cells.
Due to the presence of an NLS (nuclear localization signal), AID expression is found in both the nucleus and cytoplasm.

Applications Reported: Suitable for western blotting, immunohistochemistry (frozen and paraffin-embedded tissue), and microscopy.
Applications Tested: Effective in IHC (paraffin) using low or high pH antigen retrieval at ≤20 µg/mL; titration recommended for optimal performance.

Target Information

This gene encodes an RNA-editing deaminase belonging to the cytidine deaminase family. The protein is involved in somatic hypermutation, gene conversion, and class-switch recombination of immunoglobulin genes.
Defects in this gene cause autosomal recessive hyper-IgM immunodeficiency syndrome type 2 (HIGM2).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.