Thermo Fisher Scientific AID Monoclonal Antibody (mAID-2), Biotin, eBioscience
Human 및 Mouse AID 단백질을 인식하는 Biotin-conjugated monoclonal antibody. Western blot, IHC, Flow cytometry 등 다양한 응용에 적합. Affinity chromatography로 정제된 고순도 제품으로 안정적인 성능 제공. 연구용으로만 사용 가능.
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Thermo Fisher Scientific AID Monoclonal Antibody (mAID-2), Biotin, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-Dependent | View 5 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 20 µg/mL | - |
| Immunohistochemistry (Frozen) (IHC (F)) | 20 µg/mL | - |
| Flow Cytometry (Flow) | - | View 2 publications |
| Immunoprecipitation (IP) | - | View 1 publication |
| ChIP assay (ChIP) | - | View 1 publication |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | mAID-2 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2572797 |
| Filtration | 0.2 µm post-manufacturing filtered |
Product Specific Information
The monoclonal antibody mAID-2 recognizes human and mouse AID (gene AICDA), also known as Activation-Induced Cytidine Deaminase.
AID is a 24 kDa RNA-editing enzyme that converts cytosine into uracil and plays a critical role in B cell development, specifically in somatic hypermutation (SHM) and immunoglobulin class switch recombination (CSR).
Mutations in AID are associated with Hyper-IgM Syndrome (type 2), while overexpression correlates with poor prognosis in chronic lymphocytic leukemia/small lymphocytic lymphoma.
AID is mainly expressed by activated mature B cells (germinal centre B cells) and intermediate GC cells (IgD+CD38-CD23-CD71+). It is also found in primary human hepatocellular carcinomas, helicobacter-infected gastric epithelial cells, oocytes, and embryonic stem cells.
Due to the presence of an NLS (nuclear localization signal), AID expression is found in both the nucleus and cytoplasm.
Applications Reported: Suitable for western blotting, immunohistochemistry (frozen and paraffin-embedded tissue), and microscopy.
Applications Tested: Effective in IHC (paraffin) using low or high pH antigen retrieval at ≤20 µg/mL; titration recommended for optimal performance.
Target Information
This gene encodes an RNA-editing deaminase belonging to the cytidine deaminase family. The protein is involved in somatic hypermutation, gene conversion, and class-switch recombination of immunoglobulin genes.
Defects in this gene cause autosomal recessive hyper-IgM immunodeficiency syndrome type 2 (HIGM2).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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