Thermo Fisher Scientific CD47 Monoclonal Antibody (B6H12), NovaFluor Red 700, eBioscience

Thermo Fisher Scientific CD47 Monoclonal Antibody (B6H12), NovaFluor Red 700, eBioscience

Applications and Tested Dilution

• Flow Cytometry (Flow): 5 µL (0.8 µg)/test
• Applications Reported: Flow cytometry, immunoprecipitation, western blotting, immunohistochemistry (FFPE)
• Applications Tested: Pre-titrated for flow cytometric analysis of normal human peripheral blood cells
  • Recommended use: 5 µL (1 µg) per test (100 µL final volume)
  • Compatible cell range: 10⁵–10⁸ cells/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 700
• APC
• APC-eFluor 780
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 660-120S
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• PE-Cyanine5
• PE-Cyanine7
• PerCP-eFluor 710
• Custom conjugation available

Product Specific Information

The monoclonal antibody B6H12 reacts with CD47, also known as integrin-associated protein (IAP) and neurophilin.
CD47 is a glycosylated five-transmembrane protein involved in adhesion via interactions with SIRP and integrins (CD51/CD61, CD41/CD61). It mediates bidirectional signaling affecting neural synaptic activity and macrophage phagocytosis, and serves as a receptor for thrombospondin.

• Expression found in most hematopoietic cells (T, B cells, monocytes, platelets, erythrocytes) and non-hematopoietic tissues.
• Reported to have neutralizing activity.
• Each product includes 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer.

Usage Notes

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assays.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
  • Add 5 µL per sample or per 100 µL cell suspension (10³–10⁸ cells).
  • For antibody cocktails, add CellBlox Plus before combining with cells.

NovaFluor Technology

NovaFluor conjugates utilize Phiton™ technology, based on novel nucleic acid dye structures engineered for minimized spectral spillover and spread.

• Excitation: 639 nm
• Emission: 700 nm
• Laser: 633–640 nm (Red)

Target Information

CD47 (Integrin-associated protein, IAP) is a transmembrane glycoprotein that interacts with integrins and regulates signal transduction.
It modulates G-protein activation, calcium influx during adhesion, and serves as a receptor for thrombospondin.
CD47 binds CD172a (SIRPα) on macrophages, preventing phagocytosis of CD47-positive cells.
It influences cell migration, B cell adhesion, T cell activation, chondrocyte response to mechanical stimuli, and neuronal development, particularly in synapse-rich regions of the brain and retina.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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