Thermo Fisher Scientific BrdU Monoclonal Antibody (BU20A), FITC, eBioscience
BrdU에 특이적인 단클론 항체로 세포 내 DNA 합성 검출에 사용. FITC 형광 표지로 Flow Cytometry 및 IHC에 적합. 마우스 IgG1, kappa 아이소타입 기반. 498/517 nm 형광 특성, 4°C 암소 보관.
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Thermo Fisher Scientific BrdU Monoclonal Antibody (BU20A), FITC, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 6 publications |
| Flow Cytometry (Flow) | 5 µL (1 µg)/test | View 42 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Published Species | Chemical, Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | BU20A |
| Conjugate | FITC |
| Excitation/Emission Max | 498/517 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11043691 |
Additional Formats
- Unconjugated
- APC
- eFluor 450
- PE
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
This BU20A monoclonal antibody reacts with 5-bromodeoxyuridine (BrdU), a uridine derivative incorporated into DNA during S-phase. Anti-BrdU antibodies identify cells undergoing DNA synthesis during BrdU treatment.
For flow cytometry staining, use BrdU Staining Buffer Set (Product #00-5525) and protocol.
This antibody is validated for intracellular staining in flow cytometry and immunohistology of frozen tissue sections.
Recommended Usage
- Tested by intracellular staining and flow cytometric analysis of BrdU-labeled mouse splenocytes using Foxp3/Transcription Buffers (Product #00-5521-00) or BrdU Staining Buffer Set (Product #00-5525-00).
- Refer to BestProtocols® for intracellular antigen staining (Protocol B: One-step protocol for nuclear proteins).
- Use at 5 µL (1 µg) per test; one test defined as staining a cell sample in 100 µL final volume (10⁵–10⁸ cells/test).
- Antibody titration recommended for optimal performance.
BrdU Labeling and Staining Protocol (Flow Cytometry)
- Label dividing cells with 10 µM BrdU for 45 min at 37°C.
- Harvest and wash cells with 1X PBS.
- Perform surface staining as per protocol.
- Wash in cold Flow Cytometry Staining Buffer or PBS.
- Resuspend pellet and add 1 mL Foxp3 Fixation/Permeabilization Buffer (Product #00-5523-00).
- Incubate 30–60 min at 2–8°C in the dark.
- Wash and centrifuge; decant supernatant.
- Resuspend pellet in 100 µL Flow Cytometry Staining Buffer with 30 µg Dnase I.
- Incubate 1 hr at 37°C, then wash.
- Stain with anti-BrdU antibody for 30–60 min, wash, and analyze.
Excitation: 488 nm
Emission: 520 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside and thymidine analogue. It is incorporated into newly synthesized DNA of replicating cells during S-phase, enabling detection of proliferating cells. Anti-BrdU antibody binding requires DNA denaturation via acid or heat exposure.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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