Thermo Fisher Scientific CD24 Monoclonal Antibody (eBioSN3 (SN3 A5-2H10)), eBioscience

Applications and Tested Dilution

Product Specifications

Available Conjugate Formats

• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 805
• eFluor 450
• FITC
• NovaFluor Blue 510, 555, 585, 690, 760
• NovaFluor Red 725
• NovaFluor Yellow 570, 590, 660, 755
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436, 600, 645
• Request custom conjugation

Product Specific Information

Description:
The SN3 monoclonal antibody reacts with the human CD24 molecule, also known as Heat Stable Antigen (HSA). This 35–50 kDa molecule is anchored in the plasma membrane via phosphatidylinositol and is expressed by erythrocytes, thymocytes, peripheral lymphocytes, and myeloid lineage. It has been reported that P-selectin (CD62P) binds to CD24.

Applications Reported:
The SN3 antibody has been reported for use in flow cytometric analysis.

Applications Tested:
The SN3 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. It can be used at ≤0.5 µg per test. A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically (10⁵–10⁸ cells/test). It is recommended that the antibody be titrated for optimal performance.

Purity: Greater than 90%, as determined by SDS-PAGE
Aggregation: Less than 10%, as determined by HPLC
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD24 is a two-chain glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed at multiple stages of B-cell development, beginning with the bone marrow pro-B-cell compartment and continuing through mature, surface Ig-positive B-cells. Plasma cell expression is very low or negative. CD24 is also expressed on the majority of B-lineage acute lymphoblastic leukemias, B-cell CCLs, and B-cell non-Hodgkin’s lymphomas. It may play a role in regulation of B-cell proliferation, maturation, and autoimmunity control.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.