Thermo Fisher Scientific CD24 Monoclonal Antibody (eBioSN3 (SN3 A5-2H10)), eBioscience
인간 CD24 항원을 인식하는 eBioSN3 클론의 마우스 단클론 항체로, Flow Cytometry에 최적화되어 있습니다. 0.5 µg/test로 사용 가능하며, 높은 순도(>90%)와 낮은 응집도(<10%)를 보유합니다. 다양한 형광 및 효소 표지 형식으로 제공됩니다.
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카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD24 Monoclonal Antibody (eBioSN3 (SN3 A5-2H10)), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 2 publications |
| Flow Cytometry (Flow) | 0.5 µg/test | View 29 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioSN3 (SN3 A5-2H10) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2864852 |
Available Conjugate Formats
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 805
- eFluor 450
- FITC
- NovaFluor Blue 510, 555, 585, 690, 760
- NovaFluor Red 725
- NovaFluor Yellow 570, 590, 660, 755
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436, 600, 645
- Request custom conjugation
Product Specific Information
Description:
The SN3 monoclonal antibody reacts with the human CD24 molecule, also known as Heat Stable Antigen (HSA). This 35–50 kDa molecule is anchored in the plasma membrane via phosphatidylinositol and is expressed by erythrocytes, thymocytes, peripheral lymphocytes, and myeloid lineage. It has been reported that P-selectin (CD62P) binds to CD24.
Applications Reported:
The SN3 antibody has been reported for use in flow cytometric analysis.
Applications Tested:
The SN3 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. It can be used at ≤0.5 µg per test. A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically (10⁵–10⁸ cells/test). It is recommended that the antibody be titrated for optimal performance.
Purity: Greater than 90%, as determined by SDS-PAGE
Aggregation: Less than 10%, as determined by HPLC
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD24 is a two-chain glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed at multiple stages of B-cell development, beginning with the bone marrow pro-B-cell compartment and continuing through mature, surface Ig-positive B-cells. Plasma cell expression is very low or negative. CD24 is also expressed on the majority of B-lineage acute lymphoblastic leukemias, B-cell CCLs, and B-cell non-Hodgkin’s lymphomas. It may play a role in regulation of B-cell proliferation, maturation, and autoimmunity control.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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