Thermo Fisher Scientific CD25 Monoclonal Antibody (GLLZDMY), eBioscience
인간 CD25(IL-2 수용체 알파)에 특이적인 단클론 항체로, 활성화된 T세포 및 조절 T세포 표지를 위한 연구용 시약입니다. Western blot 및 파라핀 포매 조직 IHC에 사용 가능하며, 고순도(>90%) 및 저응집(<10%) 특성을 가집니다. 4°C 보관, PBS 완충액 포함.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-Dependent |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GLLZDMY |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2572851 |
| Purity | >90% (SDS-PAGE) |
| Aggregation | <10% (HPLC) |
| Filtration | 0.2 µm post-manufacturing filtered |
Product Specific Information
This GLLZDMY monoclonal antibody reacts with human CD25, also known as the low-affinity interleukin (IL)-2 receptor alpha. CD25 is expressed on activated T cells (including regulatory T cell subsets), B cells, and macrophages. This receptor subunit associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high-affinity IL-2 receptor complex. IL-2 receptor signaling is linked to lymphocyte proliferation and survival.
CD25 serves as a marker for Hodgkin’s disease and is associated with autoimmune conditions such as Type I diabetes.
Applications Reported:
- Western blotting
- Immunohistochemical staining of formalin-fixed, paraffin-embedded tissue sections
Applications Tested:
Tested in IHC (P) using both low and high pH antigen retrieval at 5 µg/mL. It is recommended to titrate the antibody for optimal assay performance.
Target Information
CD25 (IL2 receptor alpha chain, IL2RA) is a cytokine receptor subunit involved in T and B lymphocyte proliferation. The IL2RA, IL2RB (beta chain), and IL2RG (gamma chain) form a high-affinity IL-2 receptor complex. Homodimeric IL2RA chains yield a low-affinity receptor, while IL2RB homodimers produce a medium-affinity receptor.
IL2 expression in mature thymocytes is monoallelic, enabling precise gene regulation. IL2, primarily produced by mature T cells, functions as a growth and differentiation factor and regulates cell death. It promotes B cell proliferation, enhances NK cell activity, and inhibits granulocyte-macrophage colony formation. Disruption of the IL2RA gene in mice results in ulcerative colitis-like disease, implicating its role in immune response regulation. Mutations in IL2RA are associated with interleukin-2 receptor alpha deficiency.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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