Thermo Fisher Scientific Phospho-IKK epsilon (Ser172) Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:1,000–1:3,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:50–1:200

Product Specifications

Product Specific Information

Antibody detects endogenous levels of IKK epsilon only when phosphorylated at Ser172.

Target Information

IKBKE is an activator of nuclear factor-kappa-B (NF-κB). Overexpression of IKBKE has been observed in breast cancer cells, and suppression of IKBKE induces cell death.
NF-κB is a ubiquitous transcription factor and an essential mediator of gene expression during immune and inflammatory responses. It mediates expression of various genes in response to extracellular stimuli and is associated with IκB proteins in the cytoplasm, which inhibit NF-κB activity.
IκB is phosphorylated by the IκB kinase (IKK) complex containing IKK-α, IKK-β, and IKK-γ. A novel molecule in this complex, IKK-iota/IKK-i, was identified. IKK epsilon is required for activation of NF-κB by PMA and T cell receptors, but not by TNF-α and IL-1.
IKK-iota/IKK-i message is expressed in various tissues and is inducible by TNF-α, IL-1, and LPS.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.