Thermo Fisher Scientific NEDD4 Monoclonal Antibody (OTI7A1), TrueMAB

Applications

• Western Blot (WB): 1:500

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

The E3 ubiquitin-protein ligase NEDD4 is a Ubl conjugation pathway protein containing one HECT (E6AP-type E3 ubiquitin-protein ligase) domain and four WW domains.
It is involved in VEGFR-2/KDFR degradation pathways, acts as a potential proto-oncogene regulating PTEN via ubiquitination, and participates in viral budding processes.
NEDD4 also regulates epithelial Na⁺ channels, influencing arterial hypertension. It localizes to the sarcolemmal region of muscle fibers, where increased expression is associated with muscle unloading and reduced Notch1 expression, acting as a down-regulator of Notch-1 gene expression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.