Thermo Fisher Scientific MCFD2 Monoclonal Antibody (294301)
Human MCFD2 단백질을 인식하는 Mouse IgG2b 단클론 항체로, WB 및 IHC에 사용 가능. E. coli 유래 재조합 항원으로 제작되었으며, Protein A/G로 정제된 동결건조 형태. PBS와 trehalose buffer에 보관하며, 연구용으로만 사용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| Immunohistochemistry (IHC) | 8–25 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 294301 |
| Immunogen | E. coli-derived recombinant human SDNSF/MCFD2 (Glu27–Gln146) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.5 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No Preservative |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2606510 |
Product Specific Information
- In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse SDNSF is observed.
- In Western blots, no cross-reactivity with recombinant mouse SDNSF is observed.
- Reconstitute at 0.5 mg/mL in sterile PBS.
Target Information
Multiple coagulation factor deficiency protein 2 (MCFD2) is localized in the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) through a direct, calcium-dependent interaction with LMAN1.
The MCFD2-LMAN1 complex forms a specific cargo receptor for the transport of selected proteins from the endoplasmic reticulum to the Golgi apparatus.
Mutations in the MCFD2 gene may cause factor V and factor VIII combined deficiency (F5F8D), an autosomal recessive human bleeding disorder characterized by the reduction of both clotting proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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