Thermo Fisher Scientific Myogenin Monoclonal Antibody (F5D), eBioscience
Myogenin 단백질을 인식하는 F5D 클론의 단일클론 항체로, WB와 ICC/IF에 적합합니다. 인간, 마우스, 개, 랫드 반응성이 있으며, 근육 분화 연구에 활용됩니다. 액상 형태로 제공되며 4°C에서 보관합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–5 µg/mL | View 2 publications |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | – | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1–5 µg/mL | View 3 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rat |
| Published Species | Dog, Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | F5D |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865115 |
Additional Formats
Product Specific Information
This F5D monoclonal antibody reacts with human, mouse, dog, and rat myogenin, a 34-kDa transcription factor expressed in skeletal and heart muscle. Myogenin belongs to the MyoD family of basic-helix-loop-helix proteins, including MyoD, Myf5, and MRF4. It plays a critical role in myogenic differentiation and can direct nonmuscle cells toward the myogenic lineage. Transforming growth factor-beta (TGFβ) and bone morphogenetic protein-2 (BMP2) inhibit myogenin transcriptional activity.
Myogenin predominantly resides within the nucleus, with subcellular localization dependent on differentiation status and cell density. During skeletal muscle differentiation, myogenin trafficking between the nucleus and cytoplasm mediates transcription control.
Applications Reported: Immunoblotting (WB) and immunocytochemistry (ICC/IF)
Applications Tested: Tested in western blot and immunocytochemistry using the C2C12 cell line. Recommended concentration ≤ 1–5 µg/mL. Optimize dilution for best assay performance.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Myogenin is a muscle-specific transcription factor that induces myogenesis in diverse cell types. It belongs to the helix-loop-helix protein family and is essential for the development of functional skeletal muscle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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