Thermo Fisher Scientific Rhizopus arrhizus Monoclonal Antibody (WSSA-RA-1)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50

-

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Species Reactivity

Fungi

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

WSSA-RA-1

Immunogen

Water-soluble somatic antigens from Rhizopus arrhizus.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1960436

Product Specific Information

This clone recognizes Rhizopus arrhizus and other members of the family Mucoraceae including Absidia corymbifera and Rhizomucor pusillus, reacting strongly with the cytoplasm of hyphae and also possibly with the walls and septae, where present. It has been successfully used in immunohistochemistry for the specific and consistent in situ diagnosis of systemic bovine zygomycosis, attributed to its possible binding to a highly glycosylated moiety on non-structural components. This clone does not bind to water-soluble somatic antigens (WSSA) of Aspergillus spp.

In Western blot, this antibody detects a number diffuse band/s of between ~14-110kDa of Rhizopus arrhizus water-soluble somatic antigens. IHC staining requires protein digestion pre-treatment of paraffin sections using trypsin or pronase. Recommended postive control: lymph nodes from R. arrhizus infected cattle.

Target Information

R. arrhizus is an angio-invasive filamentous fungus. It is one of the main causative agents of systemic bovine and human zygomycosis, a worldwide and often fatal respiratory disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.