Thermo Fisher Scientific RPS3 Recombinant Rabbit Monoclonal Antibody (3D5)
RPS3 단백질을 인식하는 재조합 토끼 단클론 항체로 Western blot, IHC, ICC 등 다양한 응용 가능. 높은 특이성과 재현성을 제공하며, 동물 유래 성분이 없는 포뮬레이션. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 3D5 |
| Immunogen | Recombinant RPS3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2717285 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems are developed by cloning specific antibody DNA sequences from immunoreactive rabbits, followed by screening individual clones to select optimal candidates for production.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
WD repeat domain 5 (WDR5) is part of the WD repeat protein family involved in processes such as cell cycle progression, signal transduction, apoptosis, and gene regulation.
WDR5 (also known as BIG-3) is expressed in the developing growth plate, accelerates chondrocyte and osteoblast differentiation in vitro, and regulates osteoblast differentiation during embryonic bone development.
It interacts with the pluripotency factor Oct4/POU5F1 and is required for efficient formation of induced pluripotent stem (iPS) cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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