Thermo Fisher Scientific HSPA4 Recombinant Rabbit Monoclonal Antibody (3B3)
HSPA4 단백질을 표적으로 하는 Thermo Fisher의 재조합 토끼 단클론 항체. Western blot, IHC, ICC/IF, Flow Cytometry에 적합. 높은 특이성과 재현성, 동물 유래 성분이 없는 포뮬레이션 제공. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:10–1:100 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 3B3 |
| Immunogen | Recombinant HSPA4 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2717281 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems are developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broad immunoreactivity due to the rabbit immune repertoire
Target Information
The HSP70 family includes four highly conserved proteins: HSP70, HSC70, GRP75, and GRP78.
HSP70 expression increases in response to heat stress. HSP70 and HSC70 function in cytosolic, endoplasmic reticulum, and mitochondrial import machinery, found in both cytosol and nucleus.
GRP78 resides in the endoplasmic reticulum, assisting in protein folding and translocation.
GRP75 is located in the mitochondrial matrix and aids in folding nascent polypeptides from nuclear and mitochondrial origins.
Members of the HSP70 family are thought to act as force-generating motors, relying on ATP hydrolysis for activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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