Thermo Fisher Scientific ALPL Recombinant Rabbit Monoclonal Antibody (3G2)
ALPL 단백질을 표적으로 하는 Thermo Fisher의 재조합 토끼 단클론 항체(3G2). Western blot, IHC, ICC/IF, IP 등 다양한 응용에 적합. 높은 특이성과 재현성을 제공하며, HEK293 발현 시스템에서 생산된 동물 유래 성분 없는 항체.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | - |
| Immunohistochemistry (IHC) | - | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 | - |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 | - |
| Immunoprecipitation (IP) | 1:20–1:50 | - |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 3G2 |
| Immunogen | KLH-conjugated synthetic peptide derived from human Alkaline Phosphatase (residues 18–50) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.03% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2717283 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to rabbit immune repertoire
Purity is >95% by SDS-PAGE.
Target Information
Alkaline phosphatase (ALPL) is a homodimeric enzyme (~86 kDa) containing two zinc atoms per monomer, essential for catalytic activity at alkaline pH. It is present in both prokaryotes and eukaryotes with conserved function but structural adaptations.
Functions:
- Plays a role in liver metabolism and skeletal development
- Acts as a source of inorganic phosphate in bacteria
- Useful in yeast studies of membrane protein sorting
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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