Thermo Fisher Scientific ALPL Recombinant Rabbit Monoclonal Antibody (3G2)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

-

Immunocytochemistry (ICC/IF)

1:50-1:100

-

Immunoprecipitation (IP)

1:20-1:50

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

3G2

Immunogen

KLH conjugated synthetic peptide derived from human Alkaline Phosphatase: 18-50 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ALPL,uniProtId:P05186-1,ncbiNodeId:9606,antigenRange:18-50,antigenLength:524,antigenImageFileName:MA5-24845_ALPL_P05186-1_Rabbit.svg,antigenImageFileNamePDP:MA5-24845_ALPL_P05186-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

TBS, pH 7.4, with 50% glycerol, 1% BSA

Contains

0.03% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2717283

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Purity is > 95% by SDS-PAGE.

Target Information

Alkaline phosphatase is a homodimeric protein enzyme (86 kDa), containing two zinc atoms crucial to its catalytic function per monomer, and it is optimally active at alkaline pH environments. The enzyme is found in prokaryotes and eukaryotes, with the same general function but in different structural forms suitable to organism`s environment. ALP plays a role in liver metabolism and development within the skeleton. In bacteria, ALP acts as a source of inorganic phosphate. In yeast, ALP can be a useful protein for studying membrane protein sorting.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.