Thermo Fisher Scientific MCP-3 Polyclonal Antibody, PeproTech
Goat에서 생산된 Murine MCP-3(CCL7) 특이 다클론 항체로, Western blot과 ELISA에 최적화됨. 항원 친화 크로마토그래피로 정제되었으며, 무보존 PBS 용액 형태로 제공. 염증 및 대식세포 유인 연구용으로 적합.
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Thermo Fisher Scientific MCP-3 Polyclonal Antibody, PeproTech
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | - |
| ELISA | 0.5–2.0 µg/mL | - |
| Neutralization (Neu) | - | 1 publication |
| In vitro Assay (IV) | - | 1 publication |
| Miscellaneous PubMed (Misc) | - | 1 publication |
| Immunostaining (IS) | - | 1 publication |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host/Isotype | Goat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E. coli-derived Recombinant Murine MCP-3 (CCL7) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929288 |
Product Specific Information
AA Sequence of recombinant protein:
QPDGPNASTC CYVKKQKIPK RNLKSYRRIT SSRCPWEAVI FKTKKGMEVC AEAHQKWVEE AIAYLDMKTP TPKP
Preparation:
Produced from sera of goats immunized with highly pure Recombinant Murine MCP-3 (CCL7). Anti-Murine MCP-3 (CCL7)-specific antibody was purified by affinity chromatography employing an immobilized Murine MCP-3 (CCL7) matrix.
Sandwich ELISA:
To detect Murine MCP-3 (CCL7) by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, used with PeproTech Biotinylated Anti-Murine MCP-3 (CCL7) (500-P116GBt) as a detection antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Murine MCP-3 (CCL7).
Western Blot:
To detect Murine MCP-3 (CCL7) by Western Blot, use at 0.1–0.2 µg/mL. With compatible secondary reagents, the detection limit for Recombinant Murine MCP-3 (CCL7) is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
This is a secreted chemokine that attracts macrophages during inflammation and metastasis. It belongs to the C-C subfamily of chemokines characterized by two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, which degrades extracellular matrix components.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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