Thermo Fisher Scientific CXCL1 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilutions

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
APIANELRCQ CLQTMAGIHL KNIQSLKVLP SGPHCTQTEV IATLKNGREA CLDPEAPLVQ KIVQKMLKGV PK

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Murine KC (CXCL1).
Anti-Murine KC (CXCL1)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:

• Recommended concentration: 0.25–1.0 µg/mL (100 µL/well antibody solution)
• Use with PeproTech Polyclonal Anti-Murine KC (CXCL1) (500-P115) as capture antibody
• Detection limit: 0.2–0.4 ng/well of Recombinant Murine KC (CXCL1)

Western Blot:

• Recommended concentration: 0.1–0.2 µg/mL
• Detection limit: 1.5–3.0 ng/lane under reducing or non-reducing conditions
• Use with compatible secondary reagents

Packaging:
500-P115BT-1MG is provided as 2 × 500 µg vials.

Target Information

CXCL1, CXCL2, and CXCL3 (also known as GRO alpha, GRO beta/MIP-2 alpha, and GRO gamma/MIP-2 beta) are members of the CXC chemokine subfamily.
Mature CXCL1/2/3 proteins bind with high affinity to IL-8 receptor type B and act as potent neutrophil attractants and activators.
Processed forms GRO-alpha(4-73), GRO-alpha(5-73), and GRO-alpha(6-73) exhibit approximately 30-fold higher chemotactic activity in vitro.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.