Thermo Fisher Scientific MCP-3 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
QPDGPNASTC CYVKKQKIPK RNLKSYRRIT SSRCPWEAVI FKTKKGMEVC AEAHQKWVEE AIAYLDMKTP TPKP

Preparation:
Produced from sera of goats immunized with highly pure Recombinant Murine MCP-3 (CCL7).
Anti-Murine MCP-3 (CCL7)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Murine MCP-3 (CCL7) by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required.
This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine MCP-3 (CCL7) (500-P116G) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Murine MCP-3 (CCL7).

Western Blot:
To detect mMCP-3 by Western blot analysis, this antibody can be used at 0.1–0.2 µg/mL.
Used with compatible secondary reagents, the detection limit for Recombinant mMCP-3 is 1.5–3.0 ng/lane under reducing or non-reducing conditions.

Package:
500-P116GBT-1MG is provided as 2 × 500 µg.

Target Information

This is a secreted chemokine that attracts macrophages during inflammation and metastasis.
It belongs to the C-C subfamily of chemokines characterized by two adjacent cysteine residues.
The protein is an in vivo substrate of matrix metalloproteinase 2, which degrades extracellular matrix components.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.