Thermo Fisher Scientific CXCL1 Polyclonal Antibody, PeproTech
마우스 CXCL1(GRO alpha)에 특이적인 Rabbit Polyclonal 항체로, Western blot, ELISA, IHC 등 다양한 응용 가능. 항원 친화 크로마토그래피로 정제되었으며, 동결건조 형태로 제공. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | – |
| Immunohistochemistry (IHC) | – | 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | – | 1 publication |
| ELISA | 0.5–2.0 µg/mL | 1 publication |
| Neutralization (Neu) | – | 1 publication |
| In vitro Assay (IV) | – | 2 publications |
| Miscellaneous PubMed (Misc) | – | 2 publications |
| Immunostaining (IS) | – | 5 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Murine KC (CXCL1) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929282 |
Product Specific Information
AA Sequence of recombinant protein:
APIANELRCQ CLQTMAGIHL KNIQSLKVLP SGPHCTQTEV IATLKNGREA CLDPEAPLVQ KIVQKMLKGV PK
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Murine KC (CXCL1).
Anti-Murine KC (CXCL1)-specific antibody was purified by affinity chromatography employing an immobilized Murine KC (CXCL1) matrix.
Sandwich ELISA:
To detect Murine KC (CXCL1) by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required.
In conjunction with PeproTech Biotinylated Anti-Murine KC (CXCL1) (500-P115Bt) as a detection antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Murine KC (CXCL1).
Western Blot:
To detect Murine KC (CXCL1) by Western Blot, use at 0.1–0.2 µg/mL.
Detection limit for Recombinant Murine KC (CXCL1) is 1.5–3.0 ng/lane under reducing or non-reducing conditions.
Target Information
CXCL1, CXCL2, and CXCL3 (also known as GRO alpha, GRO beta [MIP-2 alpha], and GRO gamma [MIP-2 beta]) are members of the CXC chemokine subfamily.
Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators.
In vitro, processed forms GRO-alpha(4-73), GRO-alpha(5-73), and GRO-alpha(6-73) show approximately 30-fold higher chemotactic activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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