Thermo Fisher Scientific CXCL1 Polyclonal Antibody, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

-

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

-

View 1 publication 1 publication

ELISA (ELISA)

0.5-2.0 µg/mL

View 1 publication 1 publication

Neutralization (Neu)

-

View 1 publication 1 publication

In vitro Assay (IV)

-

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

-

View 2 publications 2 publications

Immunostaining (IS)

-

View 5 publications 5 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Murine KC (CXCL1) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CXCL1 (GRO alpha),uniProtId:P12850-1,ncbiNodeId:10090,antigenRange:25-96,antigenLength:96,antigenImageFileName:500-P115-100UG_CXCL1_GRO_alpha_P12850-1_Rabbit.svg,antigenImageFileNamePDP:500-P115-100UG_CXCL1_GRO_alpha_P12850-1_Rabbit_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2929282

Product Specific Information

AA Sequence of recombinant protein: APIANELRCQ CLQTMAGIHL KNIQSLKVLP SGPHCTQTEV IATLKNGREA CLDPEAPLVQ KIVQKMLKGV PK.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine KC (CXCL1). Anti-Murine KC (CXCL1)-specific antibody was purified by affinity chromatography employing an immobilized Murine KC (CXCL1) matrix.

Sandwich ELISA: To detect Murine KC (CXCL1) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine KC (CXCL1) (500-P115Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine KC (CXCL1).

Western Blot: To detect Murine KC (CXCL1) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine KC (CXCL1) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

CXCL1, CXCL2, and CXCL3, also known respectively as GRO alpha, GRO beta (MIP-2 alpha) and GRO gamma (MIP-2 beta), are members of the CXC subfamily of chemokines. Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.