Thermo Fisher Scientific ATM Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000–1:10,000

Immunoprecipitation (IP)

• Tested Dilution: 2–5 µg/mg lysate

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt.

Target Information

Ataxia-telangiectasia Mutated (ATM) is a protein belonging to the PI3/PI4 kinase family. Ataxia-telangiectasia is a rare autosomal recessive disorder characterized by progressive neurologic degeneration, immunologic deficiency, and increased risk of lymphoid cancer.
The ATM gene encodes a protein of the phosphoinositide 3-kinase (PI3K) superfamily. Unlike PI3K, ATM phosphorylates proteins rather than lipids and regulates cell-cycle through targets such as P53, Mdm2, BRCA1, and SMC1.
ATM plays a crucial role in repairing double-stranded DNA breaks caused by ionizing radiation. Its C-terminal region shares homology with catalytic domains of PI3 kinases. Upon ionizing radiation exposure, ATM becomes autophosphorylated and activated, triggering kinase cascades that regulate cell cycle, apoptosis, and DNA repair.
Defective ATM in AT cells results in hypersensitivity to ionizing radiation, impaired DNA synthesis inhibition, and delayed p53 induction. Studies link ATM to apoptosis through interactions with Nbs1 and Chk2 in the E2F1 pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.