Thermo Fisher Scientific ATM Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000–1:10,000

Immunoprecipitation (IP)

• Tested Dilution: 2–5 µg/mg lysate

Product Specifications

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Target Information

Ataxia-telangiectasia Mutated (ATM) is a protein belonging to the PI3/PI4 kinase family. Ataxia-telangiectasia is a rare autosomal recessive disorder characterized by progressive neurologic degeneration, immunologic deficiency, and increased risk of lymphoid cancer.
The ATM gene encodes a protein of the phosphoinositide 3-kinase (PI3K) superfamily that phosphorylates proteins rather than lipids. Its downstream targets include P53, Mdm2, BRCA1, and SMC1, which act as cell-cycle regulators.
ATM plays a crucial role in repairing double-stranded DNA breaks caused by ionizing radiation and other mutagens. The C-terminal region of ATM shows homology to catalytic domains of PI3 kinases.
ATM becomes autophosphorylated and upregulated upon ionizing radiation exposure. AT cells are hypersensitive to radiation, display impaired DNA synthesis inhibition, and delayed p53 induction. DNA damage activates ATM kinase, triggering a cascade of reactions regulating cell cycle, apoptosis, and DNA repair.
ATM is also linked to apoptosis along with Nbs1 and Chk2 in the E2F1 pathway.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.