Thermo Fisher Scientific ETS1 Recombinant Rabbit Monoclonal Antibody (JM92-32)
ETS1 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, IHC, ICC, Flow cytometry 등에 사용 가능. 높은 특이성과 민감도, 로트 간 일관성 제공. 인간 및 마우스 반응성, Protein A 정제, 액상 형태로 공급.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:5,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | - |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | - |
| Flow Cytometry (Flow) | 1:50–1:100 | - |
| Immunoprecipitation (IP) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JM92-32 |
| Immunogen | Synthetic peptide within Human ETS1 aa 10–59 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2810009 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened for optimal production.
Key advantages include:
- Superior specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the large rabbit immune repertoire
Target Information
ETS1 (c-Ets-1; v-ets erythroblastosis virus E26 oncogene homolog 1 (avian); ETS-1; EWSR2; p54) is a 441 amino acid DNA-binding protein belonging to the ETS family.
It includes:
- N-terminal pointed domain
- Central transactivation domain
- Two auto-inhibitory domains flanking a conserved C-terminal Ets domain
ETS1 interacts with factors such as PEBP2alphaA and p300/CBP, mediating transactivation of Osteopontin, Presenilin-1, Stromelysin, and Collagenase. Activated by Ras/MAPK signaling, ETS1 expression increases in resting T-cells. Its activity is modulated by AP-1, Sp-1, c-myb, and MafB.
Sp100 activates ETS1 in nuclear bodies, while interactions with EAP1/Daxx repress MMP1 and BCL2 transactivation. ETS1 also interacts with STAT6 to modulate Socs-1 expression induced by IL-4 and with GATA3 to positively regulate Tax-1 dependent IL-5 expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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