
Thermo Fisher Scientific Fascin Recombinant Rabbit Monoclonal Antibody (JM12-53)
Fascin 단백질을 표적하는 재조합 토끼 단일클론 항체로, WB, IHC, ICC, Flow 등 다양한 응용 가능. 높은 특이성과 민감도, 로트 간 일관성 제공. 액상 형태로 단기 4°C, 장기 -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:2,000 |
| Flow Cytometry (Flow) | 1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JM12-53 |
| Immunogen | Recombinant protein within Human Fascin aa 221–389 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2810005 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and screened for optimal production candidates.
Advantages include:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
Fascin organizes filamentous actin into bundles with a minimum of 4:1 actin/fascin ratio. It is involved in the assembly of actin filament bundles found in microspikes, membrane ruffles, and stress fibers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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