Thermo Fisher Scientific NFkB p52 Monoclonal Antibody (E.212.1)
NFkB p52 단백질을 특이적으로 인식하는 Rabbit Monoclonal 항체입니다. Western blot, IHC, Flow Cytometry에 사용 가능합니다. 높은 특이성과 재현성을 제공하며, Human 및 비인간 영장류 시료에 반응합니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:300–1:1,200 | - |
| Flow Cytometry (Flow) | 1:50 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Non-human primate |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | E.212.1 |
| Immunogen | Synthetic peptide corresponding to residues at the amino-terminus of human NF-kappaB2 p100/p52 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 52 µg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | <0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_10984110 |
Product Specific Information
- It is not recommended to aliquot this antibody.
- This antibody is not cross-reactive with other family members.
Target Information
The NFkB transcription factor was originally identified as a protein complex consisting of a DNA binding subunit and an associated protein. The subunit is functionally related to c-Rel p75 and Rel B p68. The p50 subunit was initially believed to be a functionally unique protein derived from the amino-terminus of a precursor designated p105.
A cDNA has been isolated that encodes an alternative DNA binding subunit of NFkB. It is synthesized as a protein expressed in various cell types and, like p105, undergoes cleavage to generate its NFkB subunit, in this case a protein designated p52 (previously referred to as p49).
In contrast to p50 derived from p105, p52 acts in synergy with p65 to stimulate the HIV enhancer in transiently transfected Jurkat cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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