Thermo Fisher Scientific SDHB (Succinate Dehydrogenase B) Recombinant Rabbit Monoclonal Antibody (SDHB, 6697R)

Applications

• Immunohistochemistry (Paraffin) (IHC (P)): Tested dilution 1–2 µg/mL

Product Specifications

Product Specific Information

• Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
• Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

Complex II of the respiratory chain is involved in the oxidation of succinate and electron transfer from FADH to CoQ. It consists of four nuclear-encoded subunits localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters forming the enzyme's iron-sulfur centers. Mutations in this gene are associated with paragangliomas and pheochromocytoma, linking mitochondrial dysfunction with tumorigenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.