Thermo Fisher Scientific SDHB (Succinate Dehydrogenase B) Recombinant Rabbit Monoclonal Antibody (SDHB, 6697R)
인간 SDHB 단백질을 인식하는 재조합 토끼 단클론 항체로, IHC(P)에서 1–2 µg/mL 농도로 사용 가능. HEK293 세포 발현, Protein A/G 정제, PBS 저장. 미토콘드리아 내 복합체 II 연구에 적합한 연구용 항체.
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Applications
- Immunohistochemistry (Paraffin) (IHC (P)): Tested dilution 1–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SDHB, 6697R |
| Immunogen | Synthetic peptide corresponding to residues 201–280 of human SDHB |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Complex II of the respiratory chain is involved in the oxidation of succinate and electron transfer from FADH to CoQ. It consists of four nuclear-encoded subunits localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters forming the enzyme's iron-sulfur centers. Mutations in this gene are associated with paragangliomas and pheochromocytoma, linking mitochondrial dysfunction with tumorigenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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