Thermo Fisher Scientific SDHB (Succinate Dehydrogenase B) Recombinant Rabbit Monoclonal Antibody (SDHB, 6697R)
Recombinant Rabbit Monoclonal Antibody targeting SDHB for IHC applications. Specific to human SDHB with high purity and reproducibility. Suitable for formalin-fixed tissue staining. Provided as liquid form, 1 mg/mL concentration, stored at -20°C or -80°C.
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Thermo Fisher Scientific SDHB (Succinate Dehydrogenase B) Recombinant Rabbit Monoclonal Antibody (SDHB, 6697R)
Applications and Tested Dilution
- Immunohistochemistry (Paraffin) (IHC (P)): 1–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SDHB, 6697R |
| Immunogen | Synthetic peptide corresponding to residues in human SDHB |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Complex II of the respiratory chain is involved in succinate oxidation and electron transfer from FADH to CoQ. The complex contains four nuclear-encoded subunits located in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and includes three cysteine-rich clusters that form the enzyme’s iron-sulfur centers. Mutations in this gene are associated with paragangliomas and pheochromocytoma, linking mitochondrial dysfunction to tumorigenesis.
Usage Notice
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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