Thermo Fisher Scientific CD150 Monoclonal Antibody (mShad150), APC, eBioscience
마우스 CD150(SLAM) 단백질을 인식하는 단일클론 항체로, APC 형광 표지되어 Flow Cytometry에 적합합니다. 활성화된 T 및 B 세포에서 발현되는 CD150 검출에 사용되며, 0.125 µg/test로 최적화되어 있습니다. 연구용으로만 사용 가능합니다.
- 카탈로그번호
- 17-1502-80
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD150 Monoclonal Antibody (mShad150), APC, eBioscience
Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 0.125 µg/test
- View 11 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2b, lambda |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | mShad150 |
| Conjugate | APC |
| Excitation/Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1582223 |
Additional Formats
- APC-eFluor 780
- eFluor 450
- FITC
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Request custom conjugation
Product Specific Information
The mShad150 monoclonal antibody reacts with mouse CD150, a ~70 kDa transmembrane glycoprotein also known as Signaling Lymphocyte Activation Molecule (SLAM). CD150 is expressed by T cells (especially Th1) and B cells, with rapid upregulation upon activation. It is also expressed by immature thymocytes and dendritic cells.
Signaling through SLAM in T cells induces proliferation and enhances the interferon-gamma response. SLAM may also mediate adhesion between T cells and antigen-presenting cells. The mShad150 antibody has been reported to stain the CD150⁺CD244⁻CD48⁻CD41⁻ population of pluripotent hematopoietic stem cells.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Tested on mouse splenocytes at ≤0.125 µg/test (100 µL final volume)
- Recommended Cell Range: 10⁵–10⁸ cells/test
- Excitation/Emission: 633–647 nm / 660 nm (Red Laser)
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD150 (SLAM, Signaling Lymphocyte Activation Molecule) is a member of the immunoglobulin superfamily involved in T cell stimulation. It is constitutively expressed on peripheral blood memory T cells, T cell clones, immature thymocytes, and some B cells, and is rapidly induced on naive T cells upon activation.
Activated B cells express membrane-bound, soluble, and cytoplasmic isoforms of SLAM. SLAM–SLAM interactions enhance B cell expansion and differentiation. The extracellular domain of CD150 acts as a receptor for the measles virus and functions as a co-activator on T and B cells. CD150 interacts with SH2D1A and PTPN11 via its cytoplasmic domain, and mutations in the CD150 gene may be associated with X-linked lymphoproliferative disease (XLP).
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.