Thermo Fisher Scientific CD150 Monoclonal Antibody (9D1), APC, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 1 µg/test
• Publications: View 8 publications

Product Specifications

Additional Formats

• Biotin
• FITC
• Functional Grade
• PE
• PE-Cyanine7
• Request custom conjugation

Product Specific Information

The 9D1 monoclonal antibody reacts with mouse CD150, a ~70 kDa transmembrane glycoprotein also known as Signaling Lymphocyte Activation Molecule (SLAM). CD150 is expressed by T (especially TH1) and B cells, and its expression increases upon activation. It is also found on immature thymocytes and dendritic cells. SLAM signaling in T cells induces proliferation and enhances interferon-gamma responses, playing a role in adhesion between T cells and antigen-presenting cells. The 9D1 antibody is reported to be activating.

Mouse hematopoietic stem cells (HSC) can be identified using SLAM family markers such as CD150⁺CD244⁻CD48⁻. For this application, the antibody clone mShad150 (Product #12-1502) is recommended.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse thymic and splenic cells
• Recommended Use: ≤1 µg/test (in 100 µL volume, 10⁵–10⁸ cells/test)
• Excitation: 633–647 nm
• Emission: 660 nm
• Laser: Red Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD150 (SLAM) belongs to the immunoglobulin gene superfamily and is involved in T cell stimulation. It is constitutively expressed on peripheral blood memory T cells, immature thymocytes, and some B cells, and is rapidly induced upon activation. SLAM signaling enhances B cell activation and differentiation and serves as a receptor for the measles virus. It interacts with SH2D1A and PTPN11 via its cytoplasmic domain, and mutations in CD150 are associated with X-linked lymphoproliferative disease (XLP).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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