Thermo Fisher Scientific Themis Monoclonal Antibody (1TMYS), eFluor 660, eBioscience
마우스 Themis 단백질을 인식하는 1TMYS 단클론 항체로, eFluor 660 형광 표지. 세포내 염색 및 유세포분석(Flow Cytometry)에 적합. 0.25 µg/test 권장. 4°C 암소 보관, 동결 금지. 연구용 시약.
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1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Themis Monoclonal Antibody (1TMYS), eFluor 660, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 0.25 µg/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 660, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1TMYS |
| Conjugate | eFluor™ 660 |
| Excitation/Emission Max | 651/668 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2574253 |
Additional Formats
Product Specific Information
Description:
The 1TMYS monoclonal antibody reacts with mouse Thymocyte-expressed molecule involved in selection (Themis), also known as Grb2-associating protein (Gasp). Themis is a cytoplasmic protein essential for positive selection and development of single-positive thymocytes. It is highly expressed in CD4+CD8+ double-positive thymocytes, with lower expression in mature T cells. Themis associates with PLCγ1 and LAT and is rapidly phosphorylated by SLP-76 following TCR signaling.
There are five isoforms of mouse Themis produced by alternative splicing. The 1TMYS antibody recognizes isoforms 1–4 but not isoform 5.
Applications Reported:
- Intracellular staining followed by flow cytometric analysis.
Applications Tested:
- Tested by intracellular staining and flow cytometric analysis of mouse thymocytes or splenocytes using the Intracellular Fixation & Permeabilization Buffer Set (Product #88-8824-00) and protocol.
- Refer to BestProtocols®: Protocol A: Two-step protocol for cytoplasmic intracellular proteins.
- Recommended use: ≤0.25 µg per test (100 µL final volume, 10⁵–10⁸ cells/test).
- Antibody should be titrated for optimal performance.
Fluorochrome Information:
eFluor® 660 is a replacement for Alexa Fluor® 647.
- Excitation: 633–647 nm
- Emission: 668 nm
- Laser: Red Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
Themis is a cytoplasmic protein essential for positive selection and development of single-positive thymocytes. It is highly expressed in CD4+CD8+ double-positive thymocytes and less in mature T cells. Themis associates with PLCγ1 and LAT and is phosphorylated by SLP-76 following TCR signaling. There are five isoforms generated by alternative splicing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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