Thermo Fisher Scientific IgM Monoclonal Antibody (II/41), eFluor 660, eBioscience
마우스 IgM 무거운 사슬을 인식하는 II/41 단클론 항체로, eFluor 660 형광체가 결합된 형광 표지 항체입니다. Flow cytometry, IHC, ICC 등 다양한 분석에 적합하며, 633 nm 레이저로 여기되고 668 nm에서 방출됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | 10 µg/mL | View 3 publications |
| Immunocytochemistry (ICC/IF) | 10 µg/mL | View 2 publications |
| Flow Cytometry (Flow) | 0.25 µg/test | View 22 publications |
| ELISA | - | View 5 publications |
| T-Cell Activation (TCA) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published species | Fish, Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 660, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | II/41 |
| Conjugate | eFluor™ 660 |
| Excitation / Emission Max | 651 / 668 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2574245 |
Additional Available Formats
- Unconjugated
- APC
- APC-eFluor 780
- Biotin
- Brilliant Violet 605
- Brilliant Violet 711
- eFluor 450
- eFluor 570
- FITC
- NovaFluor Red 710
- NovaFluor Yellow 610
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Super Bright 780
- Request custom conjugation
Product Specific Information
The II/41 monoclonal antibody reacts with the mu heavy chain of mouse IgM and does not cross-react with other mouse immunoglobulin classes (IgD, IgG, IgA). IgM is expressed intracellularly during early B-cell development and on the surface of mature B cells. Fluorochrome-conjugated II/41 can be used as a detection secondary antibody for mouse IgM.
Applications Reported: Flow cytometry, microscopy, immunohistochemistry, and immunocytochemistry.
Applications Tested:
- IHC and ICC: ≤10 µg/mL
- Flow cytometry: ≤0.25 µg/test (in 100 µL final volume, 10⁵–10⁸ cells/test)
Antibody titration is recommended for optimal performance.
Fluorochrome Information:
eFluor™ 660 replaces Alexa Fluor™ 647, excited by the red laser (633 nm) and emitting at 668 nm.
Ensure the instrument supports detection of this fluorochrome.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Mouse IgM is an immunoglobulin produced by B cells during the early immune response. It is a pentamer composed of five heavy and five light chains, playing a key role in complement activation and antigen agglutination. It is widely used in research and diagnostics such as ELISA, Western blotting, and IHC.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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