Thermo Fisher Scientific IgM Monoclonal Antibody (II/41), eFluor 660, eBioscience

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

10 µg/mL

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

10 µg/mL

View 2 publications 2 publications

Flow Cytometry (Flow)

0.25 µg/test

View 22 publications 22 publications

ELISA (ELISA)

-

View 5 publications 5 publications

T-Cell Activation (TCA)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Fish, Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 660, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

II/41

Conjugate

eFluor™ 660 eFluor™ 660 eFluor™ 660

View additional formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• Brilliant Violet 605
• Brilliant Violet 711
• eFluor 450
• eFluor 570
• FITC
• NovaFluor Red 710
• NovaFluor Yellow 610
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 780
• Request custom conjugation

Excitation/Emission Max

651/668 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2574245

Product Specific Information

Description: The II/41 monoclonal antibody reacts with the mu heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated II/41 can be used as a detection secondary for mouse IgM.

Applications Reported: This II/41 antibody has been reported for use in flow cytometric analysis, microscopy, immunohistochemical staining, and immunocytochemistry.

Applications Tested: This II/41 antibody has been tested by immunocytochemistry on fixed cells and by immunohistochemistry on formalin-fixed paraffin embedded sections that were stained first with a primary antibody of the mouse IgM isotype. For immunocytochemistry and immunohistochemistry, this can be used at less than or equal to 10 µg/mL. This II/41 antibody has also been tested by flow cytometric analysis of mouse bone marrow cells and can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody by carefully titrated for optimal performance in the assay of interest.

eFluor™ 660 is a replacement for Alexa Fluor™ 647. eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Mouse IgM is an immunoglobulin M antibody that is produced by B cells in response to an antigen. It is the first antibody produced during an immune response and is the largest immunoglobulin in the serum. Mouse IgM is composed of five heavy chains and five light chains, and it is involved in complement activation and agglutination of antigens. It plays a crucial role in the innate immune response and is important for neutralizing pathogens before the adaptive immune response is fully activated. Mouse IgM is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.