Thermo Fisher Scientific IgD Monoclonal Antibody (10.4.22), Functional Grade, eBioscience

Applications

Tested Dilution

Publications

Functional Assay (Functional)

Assay-Dependent

View 1 publication 1 publication

Product Specifications

Published species

Mouse

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), Functional Grade, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

10.4.22

Conjugate

Functional Grade Functional Grade Functional Grade

Form

Liquid

Concentration

1 mg/mL

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_2866105

Product Specific Information

Description: This 10.4.22 monoclonal antibody reacts with the a allotype of mouse IgD, which is expressed by mature peripheral B cells. This antibody has been reported to activate B cells via engagement of the IgD receptor. The use of anti-IgM as a polyclonal B cell activator is problematic due to interference from high levels of circulating serum IgM.

Crossblocking studies suggest that the 10.4.22 antibody recognizes a different epitope from 11-26c.

Applications Reported: This 10.4.22 antibody has been reported for use in functional assays.

Applications Tested: This 10.4.22 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

This type I transmembrane protein is co-expressed on the surface of mature naive B cells with membrane IgM. IgD also exists as a soluble form in blood serum. Studies have demonstrated that IgD associates with the B cell receptor and participates in mediating signal transduction upon antigen binding.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.