Thermo Fisher Scientific CD178 (Fas Ligand) Monoclonal Antibody (MFL3), Functional Grade, eBioscience

Thermo Fisher Scientific CD178 (Fas Ligand) Monoclonal Antibody (MFL3), Functional Grade, eBioscience

Applications and Tested Dilution

Product Specifications

Available Conjugated Formats

• APC
• APC-eFluor 780
• Biotin
• FITC
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

The MFL3 monoclonal antibody reacts with mouse Fas (CD95) Ligand, a 40 kDa type II transmembrane glycoprotein belonging to the TNF family. FasL is expressed by activated mouse T cells and induces Fas-mediated apoptosis through interaction with its receptor CD95.
Human FasL can be cleaved by matrix metalloproteinases (MMPs) to form a 26 kDa soluble variant; sensitivity of mouse FasL to MMPs has not been reported.

Applications Reported:

• Flow cytometric analysis
• Blocking of FasL-mediated killing in functional assays

Applications Tested:

• Tested on mouse FasL-transfected and activated T cells
• Recommended ≤ 0.5 µg/test (100 µL final volume)
• Optimal titration required per assay

Additional Information:

• Use in sterile environment
• Filtration: 0.2 µm post-manufacturing filtered
• Purity: >90% (SDS-PAGE)
• Endotoxin Level: <0.001 ng/µg antibody (LAL assay)
• Aggregation: <10% (HPLC)

Target Information

CD178 (Fas ligand, FasL) is a type-II membrane protein with its N-terminus located in the cytoplasm and C-terminus extending extracellularly. It binds to its receptor FasR, a type-I membrane protein of the TNF receptor family, to induce apoptosis.
CD178 exists in both membrane-bound (45 kDa) and soluble (26 kDa) forms, the latter generated by metalloproteinase cleavage. Binding of CD178 to Fas triggers receptor oligomerization and apoptotic signaling.

Expression and Function:

• Predominantly expressed in activated T-lymphocytes and NK cells
• Found in immune-privileged tissues (testis, eye) and organs like thymus, liver, ovary, lung, heart, and kidney
• Involved in antiviral immune responses and immune regulation
• Associated with inflammatory diseases such as atherosclerosis and hypertension
• Plays roles in pathologies including AIDS, hepatitis, and cancer

Usage Restriction:
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.