Thermo Fisher Scientific AIF Monoclonal Antibody (4E7E11)
AIF 단백질을 검출하는 Mouse 모노클로날 항체로, WB, IHC, ICC/IF, IP 등 다양한 응용에 사용 가능. Human, Mouse, Rat, Non-human primate 반응성. 1 mg/mL 농도의 액상형 제품으로 Protein A 정제, PBS 버퍼 및 sodium azide 포함. 연구용 전용.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500 | View 5 publications |
| Immunohistochemistry (IHC) | - | View 2 publications |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 | View 3 publications |
| Immunoprecipitation (IP) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4E7E11 |
| Immunogen | Purified recombinant fragment of human AIF expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11153781 |
Product Specific Information
MA5-15880 targets AIF in FACS, IF, IHC, and WB applications and shows reactivity with Human, Mouse, Non-human primate, and Rat samples.
The immunogen is a purified recombinant fragment of human AIF expressed in E. coli.
MA5-15880 detects AIF with a predicted molecular weight of approximately 67 kDa.
Target Information
Apoptosis Inducing Factor (AIF) causes chromatin condensation and DNA fragmentation. AIF localizes in mitochondria and translocates to the nucleus during apoptosis, triggering release of apoptogenic proteins such as cytochrome c and caspase-9.
AIF induces chromatin condensation and large-scale DNA fragmentation, hallmarks of apoptosis. It is highly conserved between human and mouse and widely expressed.
Mutations in the AIF gene cause combined oxidative phosphorylation deficiency 6, leading to severe mitochondrial encephalomyopathy. Alternative splicing results in multiple transcript variants, and a related pseudogene exists on chromosome 10.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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