Thermo Fisher Scientific ITK Monoclonal Antibody (5G6)
Human ITK 단백질을 인식하는 Mouse IgG1 단클론 항체로, WB, ICC/IF, Flow Cytometry, ELISA 등 다양한 응용에 적합. 비결합형 액상 형태이며 4°C 또는 -20°C에서 안정적으로 보관 가능. T세포 신호전달 연구용으로 최적화된 연구용 시약.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5G6 |
| Immunogen | Purified recombinant fragment of human ITK expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not Determined |
| Storage Buffer | Ascites |
| Contains | 0.03% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_11154453 |
Product Specific Information
MA5-15879 targets ITK in indirect ELISA, flow cytometry, Western blot, and immunofluorescence applications, showing reactivity with human samples.
The immunogen is a purified recombinant fragment of human ITK expressed in E. coli.
MA5-15879 detects ITK with a predicted molecular weight of approximately 72 kDa.
Target Information
ITK is a tyrosine kinase expressed in T-cells containing SH2 and SH3 domains. It plays a role in T-cell proliferation and differentiation.
The Tec family of non-receptor tyrosine kinases includes Tec, Itk (also known as Emt or Tsk), Btk (Atk, BPK, Emb), Bmx, Txk (Rlk), and Dsrc28C.
All members possess SH3 and SH2 domains, and except for Txk and Dsrc28C, also have pleckstrin homology (PH) and Tec homology (TH) domains in their amino termini.
Itk associates with CD28 and becomes activated following CD28 ligation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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