Thermo Fisher Scientific Goat anti-Mouse IgG (H+L) Secondary Antibody, DyLight 680
Goat anti-Mouse IgG (H+L) DyLight 680은 마우스 IgG 검출용 고감도 형광 2차 항체입니다. Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합합니다. DyLight 680 형광으로 높은 신호 대비와 낮은 배경을 제공합니다. 연구용으로만 사용됩니다.
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Thermo Fisher Scientific Goat anti-Mouse IgG (H+L) Secondary Antibody, DyLight 680
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunohistochemistry (IHC) | 1:50–1:2,000 |
| Immunocytochemistry (ICC/IF) | 4 µg/mL |
| Flow Cytometry (Flow) | 1:25–1:100 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Host/Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Purified Mouse IgG, whole molecule |
| Conjugate | DyLight™ 680 |
| Excitation/Emission Max | 676/705 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient |
| RRID | AB_614942 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Product Specific Information
Product #35518 has been successfully used in Western blot, IF, ICC, IHC, IP, and FACS applications.
This antibody reacts with the heavy chains of mouse IgG and the light chains common to most mouse immunoglobulins, but does not react with non-immunoglobulin serum proteins. It may cross-react with immunoglobulins from other species.
Store protected from light at 4°C. For extended storage, add an equal volume of glycerol (final 50%) and store at -20°C.
DyLight 680 Amax = 682 nm; Emax = 715 nm. Mole Dye/Mole Protein Ratio is lot-dependent.
Target Information
Anti-Mouse secondary antibodies are affinity-purified and specific for mouse immunoglobulins. They are useful for detection, sorting, or purification of target proteins.
Secondary antibodies enhance versatility and sensitivity by enabling multiple detection systems (HRP, AP, fluorescence) and signal amplification.
Typically generated by immunizing host animals with pooled mouse immunoglobulins, these antibodies can be further purified or modified for high specificity.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
Available Formats
Other conjugate options include:
- Agarose
- Alexa Fluor series (405, 488, 555, 647, 680, 790, Plus variants)
- DyLight series (350, 405, 488, 550, 594, 633, 650, 800 4X PEG)
- FITC, HRP, PE, TRITC, Rhodamine
- AP, Biotin, bGal, WesternDot 625
- Custom conjugation available upon request
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