
Thermo Fisher Scientific PARP1 Polyclonal Antibody
PARP1 단백질을 인식하는 Rabbit Polyclonal 항체로 Western blot, IHC, ICC, IP, ChIP 등 다양한 응용에 사용 가능. 인간 및 마우스 반응성, 항원 친화 크로마토그래피로 정제, 액상 형태로 제공되며 냉장 또는 냉동 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:20,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
| Immunoprecipitation (IP) | 1:100–1:500 | - |
| ChIP assay (ChIP) | Assay-Dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to amino acids 334–619 of PARP1 (Uniprot ID: P09874) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.48 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2552155 |
Product Specific Information
- Recommended positive controls: 293T, A431, HeLa, HepG2, HCT116, HCT116 (30 µM cisplatin treatment for 24 hr), NIH-3T3, PC-12, H1299
- Predicted reactivity: Mouse (90%), Rat (88%), Bovine (91%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Poly ADP-Ribose Polymerase (PARP) uses NAD (oxidized form) as a substrate to catalyze the transfer of ADP-ribose to various nuclear protein acceptors.
Proteolysis of PARP to its stable 85 kDa fragment is an early marker of apoptosis, mediated by the caspase CPP32 protein.
Cleavage occurs between Asp216 and Gly217, a conserved site across species.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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