Thermo Fisher Scientific PARP1 Polyclonal Antibody
PARP1 단백질을 인식하는 Thermo Fisher Scientific의 rabbit polyclonal antibody로, WB, IHC, ICC, IP, ChIP 등 다양한 응용에 적합. 고순도 항원 친화 크로마토그래피로 정제되었으며, 1 mg/mL 농도의 액상 형태로 제공. 인간 및 랫트 반응성 확인.
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Thermo Fisher Scientific PARP1 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
| Immunoprecipitation (IP) | 1:100–1:500 | - |
| ChIP assay (ChIP) | Assay-dependent | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to amino acids 34–277 of PARP1 (Uniprot ID: P09874) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2552154 |
Product Specific Information
- Recommended positive controls: 293T, A431, HeLa, HepG2, A549, PC-12, Rat2
- Predicted reactivity: Mouse (84%), Rat (85%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyze the transfer of ADP-ribose to various nuclear protein acceptors.
Proteolysis of PARP to its stable 85 kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein.
Cleavage occurs between Asp216 and Gly217, a site conserved across species.
⚠ WARNING
This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
제품 이미지
(이미지 파일: PA5-34802_PARP1_P09874-1_Rabbit.svg, PA5-34802_PARP1_P09874-1_Rabbit_PDP.jpeg)
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