Thermo Fisher Scientific CD3 Monoclonal Antibody (UCHT1), Super Bright 600, eBioscience

Applications

• Flow Cytometry (Flow)
  • Tested Dilution: 5 µL (0.5 µg)/test
  • View 8 publications

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 532, 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV / Violet series
• eFluor 450, 506
• FITC, PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• Super Bright 436, 645, 702, 780
• NovaFluor Blue, Red, Violet, Yellow series
• Functional Grade
• Request custom conjugation

Product Specific Information

Description:
The UCHT1 monoclonal antibody reacts with human CD3e, a 20 kDa subunit of the TCR complex. Together with CD3γ and CD3δ, the ε chain is essential for proper assembly, trafficking, and surface expression of the TCR complex. CD3 is expressed by thymocytes and all mature T cells. Crosslinking via UCHT1 induces intracellular signaling leading to cellular activation and proliferation.

Applications Reported:

• Flow cytometric analysis

Applications Tested:

• Pre-titrated and validated for flow cytometry using normal human peripheral blood cells
• Recommended use: 5 µL (0.5 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test)

Fluorochrome Information:
Super Bright 600 is a tandem dye excited by the violet laser (405 nm) and emits at 600 nm.
Recommended filter: 610/20 bandpass.
Use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific interactions when using multiple Super Bright dyes.

Handling and Fixation:

• Light-sensitive: protect from light
• Fixed samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333)
• Excitation: 405 nm; Emission: 600 nm; Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

The CD3 subunit complex is essential for transducing antigen-recognition signals into T cells and regulating TCR surface expression. It includes CD3γ, CD3δ, CD3ε, and CD3ζ, encoded on human chromosome 11. These subunits associate with intracellular signaling molecules via tyrosine-based motifs.
CD3 is present on 68–82% of normal peripheral blood lymphocytes, 65–85% of thymocytes, and Purkinje cells in the cerebellum, but not on B or NK cells.
Defects in the CD3 gene are associated with CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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