Thermo Fisher Scientific CD3e Monoclonal Antibody (145-2C11), Super Bright 600, eBioscience
마우스 CD3e를 인식하는 145-2C11 클론 단일클론 항체로, Super Bright 600 형광체로 결합되어 흐름세포분석에 최적화됨. T세포 표지 및 활성화 연구에 사용되며, 405 nm 레이저로 여기되어 600 nm에서 방출. 광민감성이 있어 어둡게 보관 필요.
- 카탈로그번호
- 63-0031-82
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD3e Monoclonal Antibody (145-2C11), Super Bright 600, eBioscience
Thermo Fisher Scientific CD3e Monoclonal Antibody (145-2C11), Super Bright 600, eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 7 publications |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry | 1.0 µg/test | View 41 publications |
| ELISA | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), Super Bright™ 600, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 145-2C11 |
| Conjugate | Super Bright™ 600 |
| Excitation/Emission Max | 414 / 601 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2722961 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 496, 563, 615, 661, 737
- Brilliant Violet 421, 480, 605, 650, 711, 786
- eFluor 450
- FITC
- Functional Grade
- NovaFluor Blue, Red, Yellow series
- PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436, 780
- Request custom conjugation
Product Specific Information
The 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20 kDa subunit of the TCR complex. CD3e, together with CD3γ and CD3δ, is essential for proper assembly and surface expression of the TCR complex. CD3 is expressed in thymocytes and all mature T cells. Binding of 145-2C11 to TCR initiates intracellular signaling leading to activation, proliferation, or apoptosis depending on conditions. Commonly used as a phenotypic marker for mouse T cells.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometry of mouse splenocytes; ≤1.0 µg/test recommended. A test is defined as staining in 100 µL volume with 10⁵–10⁸ cells. Titrate for optimal performance.
Fluorochrome Information:
Super Bright 600 is a tandem dye excited by the violet laser (405 nm) and emits at 600 nm. Recommended filter: 610/20 bandpass.
Usage Notes:
- Use Super Bright Complete Staining Buffer (SB-4401) when combining multiple Super Bright dye-conjugated antibodies to reduce non-specific interactions.
- Protect vial and samples from light (photo-induced oxidation sensitive).
- Fixation: Samples can be stored up to 3 days at 4°C in dark using IC Fixation Buffer (00-8222) or Fix/Lyse Solution (00-5333).
- Excitation: 405 nm; Emission: 600 nm; Laser: Violet
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
The CD3 complex transduces antigen-recognition signals in T cells and regulates TCR surface expression. It includes CD3γ, CD3δ, CD3ε, and CD3ζ subunits, encoded on chromosome 11. CD3 is expressed on 68–82% of peripheral blood lymphocytes and 65–85% of thymocytes but not on B or NK cells. CD3 defects are associated with immunodeficiency and abnormal T cell development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.