Thermo Fisher Scientific Dicer Monoclonal Antibody (N167/7), APC
Thermo Fisher Dicer Monoclonal Antibody (N167/7), APC은 인간, 마우스, 랫트에서 Dicer 단백질을 검출하는 고특이성 항체입니다. Western blot과 Immunocytochemistry에 적합하며, APC 형광 표지로 651/660 nm에서 검출됩니다. 단백질 G 정제, 4°C 보관, 연구용 전용 제품입니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N167/7 |
| Immunogen | Fusion protein amino acids 1638–1899 of mouse Endoribonuclease Dicer |
| Conjugate | APC |
| Excitation/Emission Max | 651/660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.46 mM phosphate / 2.48 mM MES, pH 7.4, with 2 mM EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932022 |
Additional Formats
- Unconjugated (MA5-27595)
- FITC (MA5-45569)
- PE (MA5-45571)
- PerCP (MA5-45570)
- Request custom conjugation
Product Specific Information
1 µg/mL of MA5-45568 was sufficient for detection of Dicer in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 215 kDa.
This antibody was formerly sold as clone S167-7.
Target Information
Dicer is a double-stranded RNA (dsRNA) endoribonuclease that plays a central role in short dsRNA-mediated post-transcriptional gene silencing. It cleaves long dsRNAs and pre-microRNAs (miRNA) into fragments of 21–23 nucleotides with 3′ overhangs, producing siRNAs and mature miRNAs. These guide the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or inhibit translation.
RNA interference via siRNAs regulates transcript elimination from repetitive DNA elements and viral RNA degradation, while the miRNA pathway specifically regulates target gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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