Thermo Fisher Scientific NFIB Recombinant Rabbit Monoclonal Antibody (7T1G5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

7T1G5

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 321-420 of human NFIB / NF1B2 (NP_0055872) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:NFIB,uniProtId:O00712-1,ncbiNodeId:9606,antigenRange:321-420,antigenLength:420,antigenImageFileName:MA5-42910_NFIB_O00712-1_Rabbit.svg,antigenImageFileNamePDP:MA5-42910_NFIB_O00712-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.24 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2912051

Product Specific Information

Positive test controls include: SH-SY5Y, Mouse lung.

Immunogen sequence: KPLFSSASPQ DSSPRLSTFP QHHHPGIPGV AHSVISTRTP PPPSPLPFPT QAILPPAPSS YFSHPTIRYP PHLNPQDTLK NYVPSYDPSS PQTSQSWYLG

Target Information

NF-1, also designated CTF, consists of a family of CCAAT box binding proteins that stimulate DNA replication and activate transcription. Analysis of human NF-1 messenger RNA has revealed two forms of the NF-1 protein arising from an alternate splicing of a single NF-1 gene. NF-1 binds its consensus DNA element as a homodimer via an amino-terminal DNA binding domain, and activates transcription through a putatively novel, proline-rich, carboxy terminal transactivation domain. The NF-1 protein has been shown to recognize and bind the adenovirus type 2 promoter and activate transcription of herpes simplex virus thymidine kinase genes. The NF-1 consensus element has been found in the upstream promoter region of myriad eukaryotic genes, including that of Ha-Ras, alpha-globin, HSP 70, GRP 78, Histone H1, myelin basic protein and in the Xenopus laevis vitellogenin gene promoter.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.